The effects of autophagy on the replication of Nelson Bay orthoreovirus.
Identifieur interne : 000197 ( Main/Exploration ); précédent : 000196; suivant : 000198The effects of autophagy on the replication of Nelson Bay orthoreovirus.
Auteurs : Xiao-Li Tao [République populaire de Chine] ; Wei Zhao [République populaire de Chine] ; Wei Tong [République populaire de Chine] ; Xiao-Fang Wang [République populaire de Chine] ; Li-Li Dou [République populaire de Chine] ; Jiang-Man Chen [République populaire de Chine] ; Nian Liu [République populaire de Chine] ; Ying Lu [République populaire de Chine] ; Yi-Bo Zhang [République populaire de Chine] ; Xu-Peng Jin [République populaire de Chine] ; Yan-Fei Shen [République populaire de Chine] ; Hong-Yan Zhao [République populaire de Chine] ; Hong Jin [République populaire de Chine] ; Yong-Gang Li [République populaire de Chine]Source :
- Virology journal [ 1743-422X ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Protéines virales.
- physiologie : Orthoreovirus.
- virologie : Infections à Reoviridae.
- Autophagie, Cellules HEK293, Charge virale, Génétique inverse, Humains, Interactions hôte-microbes, Lignée cellulaire, Réplication virale.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Viral Proteins.
- physiology : Orthoreovirus.
- virology : Reoviridae Infections.
- Autophagy, Cell Line, HEK293 Cells, Host Microbial Interactions, Humans, Reverse Genetics, Viral Load, Virus Replication.
Abstract
BACKGROUND
Nelson Bay orthoreovirus (NBV) was first isolated over 40 years ago from a fruit bat in Australia. Normally, NBV does not cause human diseases, but recently several NBV strains have been associated with human respiratory tract infections, thus attracting clinical attention. Autophagy, an evolutionarily conserved process in eukaryotic cells, degrades intracellular substrates, participates in multiple physiological processes, and maintains cellular homeostasis. In addition, autophagy is intimately involved in viral infection.
METHODS
A new strain of NBV, isolated from a patient with a respiratory tract infection who returned to Japan from Bali, Indonesia, in 2007, was used in this study. NBV was rescued using a reverse genetics system involving cotransfection of BHK cells with 11 plasmids (pT7-L1 MB, pT7-L2 MB, pT7-L3 MB, pT7-M1 MB, pT7-M2 MB, pT7-M3 MB, pT7-S1 MB, pT7-S2 MB, pT7-S3 MB, pT7-S4 MB, and pcDNA3.1-T7), yielding NBV-MB. Recovered viruses were confirmed by immunofluorescence. The effect of NBV-MB on autophagy was evaluated by measuring the LC3-I/II proteins by immunoblot analysis after infection of BHK cells. Furthermore, after treatment with rapamycin (RAPA), 3-methyladenine (3-MA), chloroquine (CQ), or plasmid (GFP-LC3) transfection, the changes in expression of the LC3 gene and the amount of LC3-I/II protein were examined. In addition, variations in viral titer were assayed after treatment of BHK cells with drugs or after transfection with plasmids pCAGM3 and pCAGS3, which encode virus nonstructural proteins μNS and σNS, respectively.
RESULTS
NBV-MB infection induced autophagy in host cells; however, the level of induction was dependent on viral replication. Induction of autophagy increased viral replication. By contrast, inhibiting autophagy suppressed NBV replication, albeit not significantly. The NBV-MB nonstructural protein μNS was involved in the induction of autophagy with viral infection.
CONCLUSIONS
NBV-MB infection triggered autophagy. Also, the NBV nonstructural protein μNS may contribute to augmentation of autophagy upon viral infection.
DOI: 10.1186/s12985-019-1196-7
PubMed: 31319897
PubMed Central: PMC6639940
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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X" first="Xiao-Fang" last="Wang">Xiao-Fang Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Dou, Li Li" sort="Dou, Li Li" uniqKey="Dou L" first="Li-Li" last="Dou">Li-Li Dou</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Jiang Man" sort="Chen, Jiang Man" uniqKey="Chen J" first="Jiang-Man" last="Chen">Jiang-Man Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Nian" sort="Liu, Nian" uniqKey="Liu N" first="Nian" last="Liu">Nian Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Ying" sort="Lu, Ying" uniqKey="Lu Y" first="Ying" last="Lu">Ying Lu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Yi Bo" sort="Zhang, Yi Bo" uniqKey="Zhang Y" first="Yi-Bo" last="Zhang">Yi-Bo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Xu Peng" sort="Jin, Xu Peng" uniqKey="Jin X" first="Xu-Peng" last="Jin">Xu-Peng Jin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Shen, Yan Fei" sort="Shen, Yan Fei" uniqKey="Shen Y" first="Yan-Fei" last="Shen">Yan-Fei Shen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Hong Yan" sort="Zhao, Hong Yan" uniqKey="Zhao H" first="Hong-Yan" last="Zhao">Hong-Yan Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang City, 110013, Liaoning Province, People's Republic of China. jinhongf@sina.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang City, 110013, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China. lygjo@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31319897</idno><idno type="pmid">31319897</idno><idno type="doi">10.1186/s12985-019-1196-7</idno><idno type="pmc">PMC6639940</idno><idno type="wicri:Area/Main/Corpus">000231</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000231</idno><idno type="wicri:Area/Main/Curation">000231</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000231</idno><idno type="wicri:Area/Main/Exploration">000231</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The effects of autophagy on the replication of Nelson Bay orthoreovirus.</title><author><name sortKey="Tao, Xiao Li" sort="Tao, Xiao Li" uniqKey="Tao X" first="Xiao-Li" last="Tao">Xiao-Li Tao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang City, 110013, Liaoning Province, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang City, 110013, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Wei" sort="Zhao, Wei" uniqKey="Zhao W" first="Wei" last="Zhao">Wei Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Tong, Wei" sort="Tong, Wei" uniqKey="Tong W" first="Wei" last="Tong">Wei Tong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Xiao Fang" sort="Wang, Xiao Fang" uniqKey="Wang X" first="Xiao-Fang" last="Wang">Xiao-Fang Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Dou, Li Li" sort="Dou, Li Li" uniqKey="Dou L" first="Li-Li" last="Dou">Li-Li Dou</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Jiang Man" sort="Chen, Jiang Man" uniqKey="Chen J" first="Jiang-Man" last="Chen">Jiang-Man Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Nian" sort="Liu, Nian" uniqKey="Liu N" first="Nian" last="Liu">Nian Liu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Lu, Ying" sort="Lu, Ying" uniqKey="Lu Y" first="Ying" last="Lu">Ying Lu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Yi Bo" sort="Zhang, Yi Bo" uniqKey="Zhang Y" first="Yi-Bo" last="Zhang">Yi-Bo Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Xu Peng" sort="Jin, Xu Peng" uniqKey="Jin X" first="Xu-Peng" last="Jin">Xu-Peng Jin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Shen, Yan Fei" sort="Shen, Yan Fei" uniqKey="Shen Y" first="Yan-Fei" last="Shen">Yan-Fei Shen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Zhao, Hong Yan" sort="Zhao, Hong Yan" uniqKey="Zhao H" first="Hong-Yan" last="Zhao">Hong-Yan Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Jin, Hong" sort="Jin, Hong" uniqKey="Jin H" first="Hong" last="Jin">Hong Jin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang City, 110013, Liaoning Province, People's Republic of China. jinhongf@sina.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang City, 110013, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China. lygjo@hotmail.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province</wicri:regionArea><wicri:noRegion>Liaoning Province</wicri:noRegion></affiliation></author></analytic><series><title level="j">Virology journal</title><idno type="eISSN">1743-422X</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Autophagy (MeSH)</term><term>Cell Line (MeSH)</term><term>HEK293 Cells (MeSH)</term><term>Host Microbial Interactions (MeSH)</term><term>Humans (MeSH)</term><term>Orthoreovirus (physiology)</term><term>Reoviridae Infections (virology)</term><term>Reverse Genetics (MeSH)</term><term>Viral Load (MeSH)</term><term>Viral Proteins (genetics)</term><term>Virus Replication (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Autophagie (MeSH)</term><term>Cellules HEK293 (MeSH)</term><term>Charge virale (MeSH)</term><term>Génétique inverse (MeSH)</term><term>Humains (MeSH)</term><term>Infections à Reoviridae (virologie)</term><term>Interactions hôte-microbes (MeSH)</term><term>Lignée cellulaire (MeSH)</term><term>Orthoreovirus (physiologie)</term><term>Protéines virales (génétique)</term><term>Réplication virale (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Orthoreovirus</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Orthoreovirus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à Reoviridae</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Reoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Autophagy</term><term>Cell Line</term><term>HEK293 Cells</term><term>Host Microbial Interactions</term><term>Humans</term><term>Reverse Genetics</term><term>Viral Load</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Autophagie</term><term>Cellules HEK293</term><term>Charge virale</term><term>Génétique inverse</term><term>Humains</term><term>Interactions hôte-microbes</term><term>Lignée cellulaire</term><term>Réplication virale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Nelson Bay orthoreovirus (NBV) was first isolated over 40 years ago from a fruit bat in Australia. Normally, NBV does not cause human diseases, but recently several NBV strains have been associated with human respiratory tract infections, thus attracting clinical attention. Autophagy, an evolutionarily conserved process in eukaryotic cells, degrades intracellular substrates, participates in multiple physiological processes, and maintains cellular homeostasis. In addition, autophagy is intimately involved in viral infection.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>A new strain of NBV, isolated from a patient with a respiratory tract infection who returned to Japan from Bali, Indonesia, in 2007, was used in this study. NBV was rescued using a reverse genetics system involving cotransfection of BHK cells with 11 plasmids (pT7-L1 MB, pT7-L2 MB, pT7-L3 MB, pT7-M1 MB, pT7-M2 MB, pT7-M3 MB, pT7-S1 MB, pT7-S2 MB, pT7-S3 MB, pT7-S4 MB, and pcDNA3.1-T7), yielding NBV-MB. Recovered viruses were confirmed by immunofluorescence. The effect of NBV-MB on autophagy was evaluated by measuring the LC3-I/II proteins by immunoblot analysis after infection of BHK cells. Furthermore, after treatment with rapamycin (RAPA), 3-methyladenine (3-MA), chloroquine (CQ), or plasmid (GFP-LC3) transfection, the changes in expression of the LC3 gene and the amount of LC3-I/II protein were examined. In addition, variations in viral titer were assayed after treatment of BHK cells with drugs or after transfection with plasmids pCAGM3 and pCAGS3, which encode virus nonstructural proteins μNS and σNS, respectively.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>NBV-MB infection induced autophagy in host cells; however, the level of induction was dependent on viral replication. Induction of autophagy increased viral replication. By contrast, inhibiting autophagy suppressed NBV replication, albeit not significantly. The NBV-MB nonstructural protein μNS was involved in the induction of autophagy with viral infection.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>NBV-MB infection triggered autophagy. Also, the NBV nonstructural protein μNS may contribute to augmentation of autophagy upon viral infection.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31319897</PMID><DateCompleted><Year>2019</Year><Month>08</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1743-422X</ISSN><JournalIssue CitedMedium="Internet"><Volume>16</Volume><Issue>1</Issue><PubDate><Year>2019</Year><Month>07</Month><Day>18</Day></PubDate></JournalIssue><Title>Virology journal</Title><ISOAbbreviation>Virol J</ISOAbbreviation></Journal><ArticleTitle>The effects of autophagy on the replication of Nelson Bay orthoreovirus.</ArticleTitle><Pagination><MedlinePgn>90</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s12985-019-1196-7</ELocationID><Abstract><AbstractText Label="BACKGROUND">Nelson Bay orthoreovirus (NBV) was first isolated over 40 years ago from a fruit bat in Australia. Normally, NBV does not cause human diseases, but recently several NBV strains have been associated with human respiratory tract infections, thus attracting clinical attention. Autophagy, an evolutionarily conserved process in eukaryotic cells, degrades intracellular substrates, participates in multiple physiological processes, and maintains cellular homeostasis. In addition, autophagy is intimately involved in viral infection.</AbstractText><AbstractText Label="METHODS">A new strain of NBV, isolated from a patient with a respiratory tract infection who returned to Japan from Bali, Indonesia, in 2007, was used in this study. NBV was rescued using a reverse genetics system involving cotransfection of BHK cells with 11 plasmids (pT7-L1 MB, pT7-L2 MB, pT7-L3 MB, pT7-M1 MB, pT7-M2 MB, pT7-M3 MB, pT7-S1 MB, pT7-S2 MB, pT7-S3 MB, pT7-S4 MB, and pcDNA3.1-T7), yielding NBV-MB. Recovered viruses were confirmed by immunofluorescence. The effect of NBV-MB on autophagy was evaluated by measuring the LC3-I/II proteins by immunoblot analysis after infection of BHK cells. Furthermore, after treatment with rapamycin (RAPA), 3-methyladenine (3-MA), chloroquine (CQ), or plasmid (GFP-LC3) transfection, the changes in expression of the LC3 gene and the amount of LC3-I/II protein were examined. In addition, variations in viral titer were assayed after treatment of BHK cells with drugs or after transfection with plasmids pCAGM3 and pCAGS3, which encode virus nonstructural proteins μNS and σNS, respectively.</AbstractText><AbstractText Label="RESULTS">NBV-MB infection induced autophagy in host cells; however, the level of induction was dependent on viral replication. Induction of autophagy increased viral replication. By contrast, inhibiting autophagy suppressed NBV replication, albeit not significantly. The NBV-MB nonstructural protein μNS was involved in the induction of autophagy with viral infection.</AbstractText><AbstractText Label="CONCLUSIONS">NBV-MB infection triggered autophagy. Also, the NBV nonstructural protein μNS may contribute to augmentation of autophagy upon viral infection.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tao</LastName><ForeName>Xiao-Li</ForeName><Initials>XL</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang City, 110013, Liaoning Province, People's Republic of China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tong</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xiao-Fang</ForeName><Initials>XF</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dou</LastName><ForeName>Li-Li</ForeName><Initials>LL</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jiang-Man</ForeName><Initials>JM</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Nian</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Ying</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yi-Bo</ForeName><Initials>YB</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Xu-Peng</ForeName><Initials>XP</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Yan-Fei</ForeName><Initials>YF</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Hong-Yan</ForeName><Initials>HY</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jin</LastName><ForeName>Hong</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, China Medical University, No. 77, Puhe Road, Shenyang North New Area, Shenyang City, 110013, Liaoning Province, People's Republic of China. jinhongf@sina.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yong-Gang</ForeName><Initials>YG</Initials><AffiliationInfo><Affiliation>Department of Pathogenic Microbiology, College of Basic Medical Sciences, Jinzhou Medical University, No. 40, the Third Section of SongPo Rd, Jinzhou City, 121200, Liaoning Province, China. lygjo@hotmail.com.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>07</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Virol J</MedlineTA><NlmUniqueID>101231645</NlmUniqueID><ISSNLinking>1743-422X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical></ChemicalList><MeshHeadingList><MeshHeading><DescriptorName UI="D001343" MajorTopicYN="Y">Autophagy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D057809" MajorTopicYN="N">HEK293 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000076662" MajorTopicYN="Y">Host Microbial Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018165" MajorTopicYN="N">Orthoreovirus</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012088" MajorTopicYN="N">Reoviridae Infections</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D059386" MajorTopicYN="N">Reverse Genetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019562" MajorTopicYN="N">Viral Load</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="Y">Virus Replication</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Autophagy</Keyword><Keyword MajorTopicYN="Y">Nelson Bay</Keyword><Keyword MajorTopicYN="Y">Orthoreovirus replication</Keyword><Keyword MajorTopicYN="Y">μNS</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate 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Jin</name><name sortKey="Jin, Xu Peng" sort="Jin, Xu Peng" uniqKey="Jin X" first="Xu-Peng" last="Jin">Xu-Peng Jin</name><name sortKey="Li, Yong Gang" sort="Li, Yong Gang" uniqKey="Li Y" first="Yong-Gang" last="Li">Yong-Gang Li</name><name sortKey="Liu, Nian" sort="Liu, Nian" uniqKey="Liu N" first="Nian" last="Liu">Nian Liu</name><name sortKey="Lu, Ying" sort="Lu, Ying" uniqKey="Lu Y" first="Ying" last="Lu">Ying Lu</name><name sortKey="Shen, Yan Fei" sort="Shen, Yan Fei" uniqKey="Shen Y" first="Yan-Fei" last="Shen">Yan-Fei Shen</name><name sortKey="Tao, Xiao Li" sort="Tao, Xiao Li" uniqKey="Tao X" first="Xiao-Li" last="Tao">Xiao-Li Tao</name><name sortKey="Tong, Wei" sort="Tong, Wei" uniqKey="Tong W" first="Wei" last="Tong">Wei Tong</name><name sortKey="Wang, Xiao Fang" sort="Wang, Xiao Fang" uniqKey="Wang X" first="Xiao-Fang" last="Wang">Xiao-Fang Wang</name><name sortKey="Zhang, Yi Bo" sort="Zhang, Yi Bo" uniqKey="Zhang Y" first="Yi-Bo" last="Zhang">Yi-Bo Zhang</name><name 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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31319897" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a RapamycinFungusV1
| This area was generated with Dilib version V0.6.38. |  |